Objective To investigate the distribution and antimicrobial resistance of clinically isolated bacteria in a hospitals from 2013 to 2018, and provide reference for rational use of antimicrobial agents in clinic. Methods Bacterial strains isolated from specimens of hospitalized patients in different clinical departments in a hospital from 2013 to 2018 were analyzed retrospectively, including the name of strains, specimen sources and antimicrobial susceptibi-lity, data were analyzed by WHONET 5.6 and SPSS 20.0 software. Results A total of 5 014 strains of bacteria were isolated in 2013-2018, 3 797 (75.73%) of which were gram-negative bacteria. The main isolated bacteria were Escherichia coli (n=1 182, 23.57%), Klebsiella pneumoniae (n=669, 13.34%), and Pseudomonas aeruginosa (n=408, 8.14%). Escherichia coli and Klebsiella pneumoniae were sensitive to carbapenems, antimicrobial resistance rates were all<10%. Resistance rates of Pseudomonas aeruginosa to imipenem and meropenem were 29.31% and 20.88% respectively. Resistance rates of Acinetobacter baumannii to imipenem and meropenem were 61.71% and 53.74% respectively. Resistance rate of Staphylococcus aureus to oxacillin was 42.14%, vancomycin and line-zolid-resistant strains was not found. Conclusion Antimicrobial resistance of clinically isolated common bacteria in this hospital is relatively stable in recent years, with an overall downward.