Objective To explore the effect of information platform construction and comprehensive intervention measures on improving pathogen detection rate of blood culture. Methods Hospitalized patients who received antimicrobial agents at a sentinel hospital in Hunan Province from January 2023 to July 2025 were selected as the research subjects. January-May 2023 was pre-intervention stage. After implementing the construction of information platform and comprehensive intervention measures, June-December 2023, January-July 2024, and August 2024-July 2025 were the first, second, and third stages of post-intervention, respectively. Blood culture detection-related indicators of hospitalized patients in 4 stages were compared. Blood culture detection rates for two important detected indicators in three stages before and after the addition of the new functional module were compared. Results After intervention, 4 indicators (the amount of blood culture detection, detection rate of standard blood culture ［bilateral double bottle］, proportion of blood culture specimens in microbial culture specimens, and blood culture positive rate) of hospitalized patients all showed gradually increasing trend, with statistical difference (all P<0.001). In the third stage after intervention, these indicators increased to 0.99 (bottle/bed·month), 54.24%, 36.35%, and 12.68%, respectively. After the addition of the new functional module, blood culture detection rates for two important indicators increased to 96.05% and 79.09%, respectively, both with statistically significant differences (both P<0.001). Conclusion The implementation of construction and continuous function re-development of the information platform, combined with comprehensive intervention measures, can sustainably and effectively improve the pathogen detection rate of blood culture.