ObjectiveTo ensure the quality safety of sterile products in regional central sterile supply department (CSSD), and promote smooth development of CSSD.Methods61 750 pieces of reusable medical instruments from 22 medical institutes which treated in a regional CSSD were divided into group A(moisture store) and group B(dry store), the rusting rate between two groups was compared, and problems in cleaning, packaging, transportation, and storage were resolved.ResultsThe rusting rate in group A was significantly higher than group B(11.71%［3 835/32 760］） vs 0.54% ［156/28 990］,χ2＝244.10，P＜0.01）. After one year training and improvement in CSSD, medical staff’s satisfaction with regional CSSD improved significantly compared that before training(90.91%［20/22］ vs 45.45%［10/22］,χ2＝13.26，P＜0.01）). The cost and expiration date of nonwoven fabrics and paperplastic wrapping material were lower and longer than cotton wrapping.ConclusionContinuous quality improvement of regional CSSD management can ensure the quality safety of supplied products and safety of medical treatment, as well as improve both social and economical effectiveness.