Abstract:ObjectiveTo establish the model about concanavalin Ainduced(Con A)experimental liver injury in mice. MethodsForty Balb/C mice were randomly divided into 4 groups(ten for each group), A, B, C and D. Con A 10mg/kg, 20mg/kg or 30mg/kg was intravenously (tail vein) injected into the mice in group A, B and C respectively while normal saline was used for the group D (control group). Activity of alanine transaminase(ALT), death rate and liver histopathological changes were observed after 8 hours of injection. The optimal Con A dose (20mg/kg) was selected, activity of ALT, death rate and liver histopathological changes were observed based on such dosage. ResultsAfter 8 hours of injection of Con A, no dead mouse was found in group A and D, but 3 and 10 mice were dead in group B and C respectively; the ALT level in group A and B was (215.55±70.19) IU /L and (2 516.14±764.69) IU / L respectively, both were significantly higher than (57.30±12.21) IU /L in group D (t=-8.466, t=-10.143, respectively; both P=0.000). Liver histopathology showed only degeneration of liver cells was present in group A, while degeneration, necrosis and liver inflammatory cells infiltration in group B and C. Within 24 hours of injection of 20mg/kg of Con A, the death rate, activity of ALT, liver inflammatory cells infiltration, hepatocyte degeneration and necrosis exacerbated with the prolongation of time. ConclusionThe optimal dosage to establish Con Ainduced experimental liver injury in mice is 20mg/kg, with the optimal observation time at 8 hours after intravenous (tail vein) injected.