刀豆球蛋白A所致实验性肝损伤模型的构建
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范学工

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R575.1

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国家自然科学基金(30671845);湖南省卫生厅科研基金(B2005030);教育部高校博士点基金新教师资助项目(20070533009)


Development of the model about concanavalin Ainduced experimental liver injury in mice
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    摘要:

    目的建立刀豆球蛋白A(Con A)诱导小鼠实验性肝损伤的模型。方法Balb/C小鼠40只,分别尾静脉注射Con A,根据注射剂量,小鼠被随机分为4组(每组10只):A组10 mg/kg;B组20 mg/kg;C组30 mg/kg;另设D组作为实验对照组,尾静脉仅注射生理盐水。给药后8 h观察小鼠血清丙氨酸转氨酶(ALT)活性、死亡率和肝组织的病理改变。选择最佳Con A剂量,观察此剂量下不同时间点ALT活性、死亡率和肝组织的病理改变特点。结果经Con A处理后8 h,A组与D组无小鼠死亡,B组死亡3只,C组小鼠全部死亡;各组血清ALT值:A组为(215.55±70.19)IU/L,B组为(2 516.14±764.69)IU/L,均显著高于对照组D组的(57.30±12.21)IU/L(分别t=-8.466、t=-10.143,均P=0.000)。光镜下,A组可见肝细胞变性;B组和C组可见肝组织炎症细胞浸润和肝细胞变性、坏死,以C组为甚。20 mg/kg Con A尾静脉注射,小鼠死亡率、ALT活性、肝组织炎症细胞浸润和肝细胞变性、坏死在一定时间范围内(24 h)随时间增加而加剧。结论Con A小鼠实验性肝损伤模型建立,Con A剂量以20 mg/kg、观察时间以8 h为宜。

    Abstract:

    ObjectiveTo establish the model about concanavalin Ainduced(Con A)experimental liver injury in mice. MethodsForty Balb/C mice were randomly divided into 4 groups(ten for each group), A, B, C and D. Con A 10mg/kg, 20mg/kg or 30mg/kg was intravenously (tail vein) injected into the mice in group A, B and C respectively while normal saline was used for the group D (control group). Activity of alanine transaminase(ALT), death rate  and liver histopathological changes were observed after 8 hours of injection. The optimal Con A dose (20mg/kg) was selected, activity of ALT, death rate  and liver histopathological changes were observed based on such dosage. ResultsAfter 8 hours of injection of Con A, no dead mouse was found in group A and D, but 3 and 10 mice were dead in group B and C respectively; the ALT level in group A and B was (215.55±70.19) IU /L and  (2 516.14±764.69) IU / L respectively, both were significantly higher than (57.30±12.21) IU /L in group D (t=-8.466, t=-10.143, respectively; both P=0.000). Liver  histopathology showed only degeneration of liver cells was present in group A, while degeneration, necrosis and liver inflammatory cells infiltration in group B and C. Within 24 hours of injection of 20mg/kg of Con A, the death rate, activity of ALT, liver inflammatory cells infiltration, hepatocyte degeneration and  necrosis  exacerbated with the prolongation of time. ConclusionThe optimal dosage to establish Con Ainduced experimental liver injury in mice is 20mg/kg, with the optimal observation time at 8 hours after intravenous (tail vein) injected.

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刘悦晖,范学工,李宁,等.刀豆球蛋白A所致实验性肝损伤模型的构建[J]. 中国感染控制杂志,2008,7(5):397-301.
LIU Yuehui, FAN Xuegong, LI Ning, et al. Development of the model about concanavalin Ainduced experimental liver injury in mice[J]. Chin J Infect Control, 2008,7(5):397-301.

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  • 收稿日期:2008-06-10
  • 最后修改日期:2008-08-12
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