ObjectiveTo investigate the distribution  and drugresistance of pathogens in  positive blood culture, so as to provide reference for the rational use of antimicrobial agents. MethodsFrom January 1, 2007 to December 31,2007, a total of 2 317 blood samples were cultured by  BACTEC9120 system, the isolates were identified by API system, drug sensitive tests were performed by KirbyBauer test, the data were analyzed by WHONET5.3 software.ResultsTwo hundred and twentythree patients in 55 clinical wards had 324 positive blood culture, the positive rate was 13.98%. The isolated strains include 26 species of bacteria and 3 species of fungi, there were 223 pathogenic strains in total. Grampositive cocci, gramnegative bacilli and Candida were 109(48.88%) strains, 106(47.53%) and 8(3.59%) respectively; The main  pathogenic bacteria were Coagulasenegative staphylococcus (67 strains), Escherichia coli (41), Klebsiella pneumoniae (20) and Staphylococcus aureus (15). The percentage of isolated bacteria in intensive care unit, emergency department and hematological department was 21.97%, 14.80% and 14.35% respectively. Methicillinresistant Coagulase negative staphylococcus (MRCNS) and methicillinresistant Staphylococcus aureus (MRSA) accounted for 73.13% and 40.00% respectively. The detection rate of extendedspectrum βlactams (ESBLs) of Escherichia coli and Klebsiella pneumoniae was  41.46% and 30.00% respectively; Drugresistant rate of MRCNS, MRSA and ESBLsproducing strains were all high.ConclusionThere are varieties of  pathogens isolated from  blood culture, and the drugresistance of pathogens are high, clinicians should pay attention to the culture  of blood  in patients suspected septicemia, so as  to use antimicrobial agents rationally.