ObjectiveTo investigate the carrying condition and  antimicrobial  susceptibility of methicillinresistant Staphylococcus (MRS) on medical staff's hands.MethodsOne hundred and sixtyeight  medical staffs were selected randomly in a hospital, 336 swabs samples were collected from their hand skin before and after “sixstep” hand washing respectively. The samples were prepared for isolated culture of MRS as well as antimicrobial susceptibility tests. ResultsThe carrying rate of  MRS on medical staffs’ hands was 66.67% and  41.07% before and after hand washing respectively, there was significant difference between the two (χ2=22.14, P<0.01). All of MRS were methicillinresistant Coagulase negative staphylococcus (MRCNS), methicillinresistant Staphylococcus aureus (MRSA) wasn’t found. Except the relatively lower drugresistant rates to vancomycin and nitrofurantoin, drugresistance rates were all high  to  penicillin, ampicillin, amoxicillin/clavulanic acid，cefalotin and cefotaxime(70.37%～100.00%); the resistant rate to ciprofloxacin, erythromycin, clindamycin, trimethoprimsulfamethoxazole was 42.33%～80.42%; The drug resistance rate of MRS was obviously higher than that of methicillinsensitive Staphylococcus, and the multiply drug resistance was observed. ConclusionHigh carrying rates of MRS on medical staff’s hands should be paid more attention. Sixstep hand washing can  reduce the carrying rates of MRS as well as  the spread and prevalence of MRS among hospital.