ObjectiveTo analyze the  data of antimicrobial resistance surveillance in a  hospital in 2007. MethodsBacterial isolates were identified by API system, antimicrobial  susceptibility  tests were performed by KirbyBauer method and  analyzed by WHONET5．4 software. ResultsA total of  3 107 clinical isolates were isolated in 2007，60.93% were gramnegative bacteria,27.78% were grampositive bacteria and 11.30％ were fungi. 60.58％of Staphylococcus aureus was methicillinresistant Staphylococcus aureus  (MRSA) and 77.35% of coagulase negative Staphylococcus was methicillinresistant coagulase negative Staphylococcus (MRCNS) respectively. The prevalence and drugresistance of Enterococcus faecium were higher than Enterococcus faecali (P<0.05), the prevalence of highlevel aminoglycosidesresistant Enterococcus was 59.44%. The prevalence of extendedspectrum  betalactamase of Escherichia coli and Klebsilla spp. was 59.50％and 55.98%, respectively. 63.97% of Pseudomonas aeruginosa were isolated from sputum, drugresistant rate  of Pseudomonas aeruginosa in sputum from patients in ICU, neurosurgical department, respiratory and emergency pediatric department, respiratory department was 21.05%, 15.99%,7.09% and 6.07% respectively .ConclusionPeriodical summarization of   antimicrobial resistance surveillance results  and  discovery of   drugresistance trends is great significant for guiding rational antimicrobial therapy.