ObjectiveTo understand and establish the normal reference values of peripheral blood CD3+, CD4+ and CD8+ T lymphocytes in healthy Miao ethnics in Hunan Province. MethodsThree hundred and sixty healthy  Miao ethnics were divided  into 5 age groups (10~19, 20~29, 30~39,40~49, 50~59), their  peripheral blood CD3+, CD4+ and CD8+ T lymphocytes were detected with FACSCalibur flowcytometry. ResultsThe mean absolute values of peripheral blood CD3+, CD4+, CD8+T lymphocytes and CD4+/CD8+ in healthy  Miao ethnics in 10～59 age groups were (1 426.71±462.92) cells/μL, (787.54±258.36) cells/μL, (589.86±242.91) cells/μL and 1.46±0.50 respectively, the mean absolute values of peripheral blood CD3+ and CD8+T lymphocytes in 10~19 age group were significantly higher than those in 30~59 age group (F=10.80,8.51, respectively, all P<0.05), CD4+T lymphocytes value was obviously higher than that in 50～59 age group (F=7.03, P＜0.05); There was on significant difference in above values between different genders (t=0.98, 0.80, 0.88, 0.16 respectively, all P＞0.05). ConclusionThe establishment of normal reference values of peripheral blood CD3+, CD4+ and CD8+T lymphocytes for healthy Miao ethnics will help to clinical diagnosis.