ObjectiveTo investigate the clinical and bacteriological characteristics of the death of patients with septicemia.MethodsClinical and experimental data of 240  patients with septicemia （29 patients died from septicemia） in a hospital in 6 years were analysed retrospectively. ResultsSixtytwo species of pathogens were isolated from 240 patients, the main pathogens were Coagulase negative Staphylococcus (CNS), which accounting for 27.92%; among 29 patients died from septicemia, the majority were septicemia caused by gramnegative bacilli (41.38% were Escherichia coli), which accounting for 65.52%, the next were CNS (20.69%). Among patients died from septicemia, male patients were more than female patients （χ2=5.29, P<0.05）, age≥15 years old were more than age＜15 years old （χ2=5.59, P<0.05）, nosocomial infections were more than communityacquired infections （χ2=43.94, P<0.01）. ConclusionCNS are the main pathogens causing septicemia, but Escherichia coli of gramnegative bacilli is still the main pathogen. Among patients died from septicemia, the predominant are adults, male patients and patients with nosocomial infection.