ObjectiveTo monitor and evaluate  disinfection and sterilization quality in medical institutions in  different levels of   hospitals in  Zhuzhou city between 2003—2008, so as  to improve infection control management.MethodsOnthespot sampling and laboratory examination methods were used to carry out survey of the medical institutions, samples included air, surface of objects, inuse disinfectants, hospital contaminated water, sterile medical instruments.The comprehensive qualities of the hospitals of classⅠandⅡwere evaluated by TOPSIS based on coefficient of entropy. ResultsThe total qualified rate of disinfection and sterilization in medical institutions in the city between 2003—2008 was 91.79%, which rose year by year (χ2=66.45, P=0.00); There was significant difference in the total qualified rate of disinfection and sterilization between different levels of hospitals(χ2=60.14, P=0.00), the lowest rate was in individual clinics; The qualified rate of inuse disinfectant, transfusion material, autoclaves were all >90.00%. There were 4 levels in the order of the  evaluated objects, the constituent ratio in 4 levels was different.ConclusionThe disinfection and sterilization  quality in the medical institutions in the city  improved year by year, but some technical advices should be given, especially for individual medical institutions.