ObjectiveTo investigate the disinfection and sterilization state of equipment, personal protection and instrument in institutions of oral cavity treatment in Hangzhou city. MethodsQuestionnaires about all oral institutions in the city were made and filled out, and onthespot samples were taken. ResultsTwo hundred and sixty one oral cavity treatment institutions were checked, 127 of which were stomatology departments in general hospitals, 134 were outpatient departments in oral cavity clinics. The qualified rate of rational distribution and supply of medical instrument of oral cavity clinics was 91.04% and 97.01% respectively, which were higher than 73.23% and 87.40% in  stomatology departments in general hospitals (χ2=14.26，P＝0.000；χ2＝8.52，P＝0.004, respectively); 79.53% of general hospitals provided goggles, which was higher than 62.69%  in outpatient departments in oral cavity clinics (χ2=8.96, P=0.003); 83.46% of stomatology departments in general hospitals performed hand washing and changed gloves between patients when medical treatment were undertaken, which was lower than 100.00% of outpatient departments (χ2=24.09, P=0.000); The qualified rate of hand hygiene of health care workers and sterilized objects in general hospitals was 71.23% and 65.38% respectively, which were higher than 54.10% and 32.50%  of oral cavity clinics (χ2=4.21,P=0.004; χ2=9.78, P=0.002, respectively). ConclusionThe disinfection and sterilization state of oral cavity equipment, personal protection and  instruments in different level oral institutions are different, it is important to strengthen the administration.