ObjectiveTo study resistance of clinical isolated Neisseria gonorrhoeae to ciprofloxacin in Nanning area. MethodsNine hundred and seventy strains of Neisseria gonorrhoeae isolated from urogenital system in patients in a dermatology institute between January 1996 and December 2008, minimum inhibitory concentrations (MICs) of ciprofloxacin were determined by agar dilution method. ResultsThe total resistant rate of Neisseria gonorrhoeae to ciprofloxacin was 81.96%, there was obviously increased tendency in resistant rate (χ2=394.49, P<0.005) with each year; drug resistant rate in 1996, 2000, 2005 and 2008 was 19.56%, 93.81%,98.75% and 100.00% respectively, drug resistant rate increased rapidly before 1999, and remained high level after 2000. The MIC50 increased from  0.125mg/L in 1996 to 4mg/L in 2000 and 8mg/L in 2006; MIC90 was also at high level. ConclusionResistance of Neisseria gonorrhoeae to ciprofloxacin is very serious in Nanning area,  fluoroquinolones such as ciprofloxacin is not suitable as the  firstline drugs for treating patients with gonorrhea in Nanning area, it is important to survey successively antimicrobial resistance of Neisseria gonorrhoeae.