ObjectiveTo investigate infections with double and multiple drugresistant bacteria in hospitalized patients, and to put forward control measures. MethodsMedical histry of patients who were hospitalized between January, 2007 and December, 2008 were analysed retrospectively. ResultsAmong 55 084 patients, 1 436 (2.61%)  developed infections with  double and multiple drugresistant bacteria, 959 (1.74%) of which were communityacquired infections, 477(0.87%) were nosocomial infections, there was significant difference between the two (χ2=323.571, P=0.000). Infection with double and multiple drugresistant bacteria accounted for 25.74% (1 853 cases) of  patients with nosocomial infection. Infection mainly occurred in patients in intensive care unit (16.98%), department of neurosurgery (14.67%), burn unit(14.25%) and department of neurology (14.04%). The main infection sites were respiratory tract (824 cases, 51.92%). 75.97% (1 091 cases) of patients  received antimicrobial agents before developing infection.ConclusionInfections with double and multiple bacteria are mainly communityacquired infection. Rational appplication of antimicrobial agents are the main strategies for controlling and reducing infection with double and multiple bacteria