To evaluate the antifungal activities of amphotericin B (AMB) combination with  azithromycin (AZI) and other antifungal agents  against 30 strains of Aspergillums fumigatus in vitro, so as to guide rational use of antifungals against invasive aspergillosis. MethodsThe M38A method recommended by Clinical and Laboratory Standards Institute was used to detect  antifungal activity of the combination application of antifungal drugs. ResultsThe minimum inhibitory concentration(MIC) of voriconazole(VRC), caspofungin(CBF), itraconazole(ICZ) and AMB against Asperillus fumigatus was 0.29 μg/mL,  0.45 μg/mL, 0.52 μg/mL and 0.55 μg/mL, respectively. Asperillus fumigatus were all resistant to terbinafine, fluorocytosin 5 (5FC),  fluconazole(FCZ) and AZI. The total percentages of synergistic activity for AMB+ICZ, AMB+VRC, AMB+CBF, and AMB+5FC was 96.67%, 90.00%, 83.33% and 50.00% respectively. ConclusionAspergillus fumigatus were highly sensitive to AMB, ICZ, VRC and CBF, but resistant to AZI and FCZ; The combination of AMB+ICZ, AMB+VRC, AMB+CBF and AMB+5FC  displayed a  synergistic activity. The combination of AMB and other antifungals  can be chosen for invasive aspergillosis.