ObjectiveTo realize the distribution and drug resistance of nonfermentative bacteria isolated from clinic, so as to provide reference for  antimicrobial use in clinic. MethodsData of 990 strains of nonfermentative bacteria isolated from clinical departments between January, 2004 and December, 2008 were  analyzed retrospectively. ResultsNine hundred and ninety nonfermentative bacteria strains were divided into 11 genera, most were Acinetobacter spp., which accounting for 43.43% (430/990); the next were Pseudomonas spp. and Stenotrophomonas spp., which was 34.75% (344/990) and 10.71% (106/990) respectively. The most common isolated bacteria were Pseudomonas aeruginosa (29.19%), Acinetobacter lwoffii (20.61%), Acinetobacter calcoaceticusbaumannii complex (13.74%) and Stenotrophomonas maltophilia (10.71%). Most strains were isolated from sputum, which accounting for 79.80% (790/990), the next were throat swab and body fluid, which was 7.88% (78/990) and 6.87% (68/990) respectively. During the recent 5 years, the difference in drug resistance of Pseudomonas aeruginosa to ticarcillin, cefoxitin, cefotaxime, imipenem, netilmicin, trimethoprim/sulfamethoxazole,  pefloxacin, gentamycin, nitrofurantoin and tobramycin  were significant (P<0.005 or P<0.05), drug resistant rates <30% were ceftazidine and imipenem; the change in drug resistance of Acinetobacter spp. to amoxicillin/clavulanic acid, gentamycin, tobramycin and trimethoprim/sulfamethoxazole were significant (P<0.005 or P<0.05), drug resistant rates <30% were ticarcillin, imipenem  and netilmicin. ConclusionNonfermentative bacteria are main pathogens in nosocomial infection, drug resistant rates to commonly used antimicrobial agents are high, and multidrug resistance are serious, periodical monitor of change in bacteria and drug resistance should be carried out to guide rational use of antimicrobial agents in clinic.