ObjectiveTo investigate the effect of suppressive oligodeoxynucleotide (Sup ODN) on pSTAT4, pSTAT6 and Tbet expression of concanavalin A induced liver injury in mice.MethodsForty Balb/C mice were randomly divided into 4 groups, normal saline group (NS, blank control group), cyclosporin A group (CsA group, positive control group) ,control oligodeoxynucleotide group (control ODN group), and Sup ODN group (trial group). Mice in each group were injected with ConA(20mg/kg) via the tail vein to make liver injury model. Sup ODN, Con ODN, CsA or NS was injected intraperitoneally at the same time of ConA injection. The expression of pSTAT4 ,pSTAT6 and Tbet in spleen at the 8th or 24th hour after intraperitoneal injection was examined by Westernblotting.ResultspSTAT4 and Tbet in Sup ODN group were significantly lower compared with NS，Con ODN or CsA group respectively ( all P< 0.01); pSTAT6 had no difference between Sup ODN group and other groups ( all P< 0.01).ConclusionSup ODN attained protective effect on ConAinduced mice liver injury by supressing expression of  pSTAT4 and Tbet.