Objective   To understand the colonization and resistance characteristics of multidrugresistant bacteria  isolated from ICU patients. Methods  Nasal vestibular  and rectal swabs of 82 patients  admitted in a hospital ICU from November, 2008 to April, 2009 were screened by bacteriological surveillance method, and antimicrobial susceptibility were performed. Data were analyzed by WHONET5.4 software. Results  Multidrugresistant strains were detected among 38 patients , the colonization rate was 46.34%, which including 62 strains of extendedspectrum β lactamases (ESBLs) producing Enterobacteriaceae bacteria and 4 strains of methicillinresistant Staphylococcus aureus(MRSA),  and multidrugresistant Acinetobacter baumannii and Pseudomonas aeruginosa was not detected. 62  strains of ESBLsproducing strains had lower drugresistant rates (4.84%)  to imipenem and meropenem,but had high sensitive rates to the other antimicrobial agents, resistant rates varied with different  bacteria; 4  MRSA strains were all sensitive to vancomycin, teicoplanin and linezolid, some were sensitive to clindamycin, fosfomycin and gentamicin, and all were resistant to other antimicrobials.Conclusion  Colonization rate of multidrugresistant bacteria is high in patients in  ICU, and drugresistance is serious, which should be paid attention by clinicians , surveillance should be intensified , so as to avoid the outbreak of multidrugresistant bacteria infection in hospital.