ObjectiveTo get information about pathogenic distribution and bacterial resistance .MethodsData about bacteria isolated from inpatients who were hospitalized  between January 1st, 2006 to  December 31st, 2008 were collected and analysed with WHONET 5.4 software.Results5 697 pathogenic bacteria were isolated within 3 years,  which included 3 627 (63.67%) gramnegative and 1 536（26.96%）grampositive strains. Escherichia coli (895 strains,15.71%), Pseudomonas aeruginosa (873 strains, 15.32%), Klebsiella pneumoniae (446 strains,7.83%), Acinetobacter baumannii(366 strains, 6.43%) were the most common gramnegative isolates; Staphylococcus aureus(648 strains,11.37%), Enterococcus spp.(373 strains, 6.55%), and coagulasenegative Staphylococcus(349 strains,6.13%) were the top three grampositive isolates. The prevalence of extended spectrum βlactamases ( ESBLs) producing bacteria, methicillinresistant Staphylococcus aureus (MRSA) and methicillinresistant coagulasenegative Staphylococcus(MRCNS)  increased year by year. Bacteria were isolated from various specimens, the top three were  sputum (45.97%), secretion (16.54%) and urine (10.65%). Resistant rates of Escherichia coli, Klebsiella pneumoniae to carbapenems were all 0.00%, but 23.85%~31.76% of Pseudomonas aeruginosa isolates were carbapenemsresistant. Less than 15.52% of the above four kinds of bacteria were resistant to cefoperazone/ sulbactam, but they were highly resistant to other antimicrobial agents; more than 70% of Escherichia coli were quinoloneresistant. No Staphylococcus were detected to be resistant to glycopeptides, such as vancomycin and linezoid , but the resistant rates to the other commonly used  antimicrobial agents were relatively high, more than 78.57% Staphylococcus were clindamycinresistant. ConclusionPathogenic bacteria are mainly isolated from sputum, secretion and urine， the main pathogens are gramnegative bacteria； Antimicrobial resistance is very severe in hospitalized patients. Surveillance of antimicrobial resistance is important and valuable for rational antimicrobial therapy.