产ESBLs大肠埃希菌氨基糖苷类修饰酶基因的检测    FREE
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黄永茂

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R378.2+1

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四川省重点学科建设项目资助(SZD0241)


Detection of aminoglycosidemodifying enzyme genes in extendedspectrum βlactamasesproducing strains of Escherichia coli    FREE
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    摘要:

    目的了解某地区产超广谱β内酰胺酶(ESBLs)大肠埃希菌氨基糖苷类修饰酶(AMEs)基因存在状况及耐药情况。方法对临床分离的75株大肠埃希菌用表型确证试验检测ESBLs, KB纸片扩散法对6种氨基糖苷类抗生素做药敏试验,并采用聚合酶链反应(PCR)检测AMEs基因。结果75株大肠埃希菌中共检出产ESBLs菌37株(49.33%)。产ESBLs菌对氨基糖苷类抗生素耐药率(包括中介株):庆大霉素78.38%,链霉素75.68%,卡那霉素67.57%,妥布霉素64.86%,奈替米星24.32%,阿米卡星13.51%;共检出5种基因,其中以aac(3)Ⅱ(64.86%)和aac(6′)Ⅰ(45.95%)为主,其次为ant(3")Ⅰ(29.73%)、ant(2")Ⅰ(10.81%)、aac(3) Ⅰ(5.41%),未检出aac(6′)Ⅱ;除ant(2")Ⅰ和aac(3)Ⅰ外,其余3种基因检出率均高于非产ESBLs菌株,且2个基因携带率也明显高于非产ESBLs菌株(P<0.05)。结论该地区产ESBLs大肠埃希菌携带AMEs基因的比率较高,对氨基糖苷类抗生素的耐药率亦高,应加强监测与防控。

    Abstract:

    ObjectiveTo detect aminoglycosidemodifying enzyme (AMEs) genes and drugresistance among extendedspectrum βlactamases (ESBLs)producing Escherichia coli (E.coli) in an area.MethodsESBLsproducing strains were detected by confirmatory test in 75 strains of E.coli; the susceptibility of 75 strains to 6 kinds of aminoglycosides (AGs) were detected by disk agar diffusion method; and the genotypes of AMEs genes were detected by PCR.ResultsIn 75 strains of E.coli, 37 (49.33% ) ESBLsproducing strains were confirmed. The resistant rates to AGs in ESBLsproducing strains were as follows: gentamycin 78.38%, streptomycin 75.68%, kanamycin 67.57%, tobramycin 64.86%, netilmicin 24.32%, and  amikacin 13.51%; Five kinds of AMEs genes were detected in ESBLsproducing strains and the main genes were aac(3)Ⅱ(64.86%) and aac(6′)Ⅰ(45.95%); the next were ant(3")Ⅰ(29.73%), ant(2")Ⅰ(10.81%) and aac(3) Ⅰ(5.41%), aac(6′)Ⅱ wasn’t  found. Except ant(2")Ⅰand aac(3)Ⅰ, the detection rates of the other 3 genes in ESBLs strains were higher than that of nonESBLs strains, and the carrying rates of 2 kinds of gene were also higher than nonESBLs strains (P<0.05). ConclusionThe carrying rates of  AMEs in ESBLsproducing E.coli are high in this area, and resistant rates to AGs are also high, the monitor should be intensified.

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黄永茂,游春芳,张馨琢,等.产ESBLs大肠埃希菌氨基糖苷类修饰酶基因的检测    FREE[J]. 中国感染控制杂志,2010,9(4):231-234.
HUANG Yongmao, YOU Chunfang, ZHANG Xinzhuo, et al. Detection of aminoglycosidemodifying enzyme genes in extendedspectrum βlactamasesproducing strains of Escherichia coli    FREE[J]. Chin J Infect Control, 2010,9(4):231-234.

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  • 收稿日期:2009-12-15
  • 最后修改日期:2010-02-10
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  • 在线发布日期: 2010-07-30
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