ObjectiveTo analyze the homology of 8 strains of Comamonas acidovorans (C. acidovorans ) isolated from a clinical department of a hospital, and evaluate the routes of bloodstream infections caused by C. acidovorans.MethodsEight strains of C. acidovorans were isolated from samples collected from patients and medical devices. C. acidovorans were identified with biochemical analysis, and their homology were determined with randomly amplified polymorphic DNA (RAPD) .ResultsEight strains of C. acidovorans had the same genotype, no C. acidovorans was isolated from hospital environment and medical devices.ConclusionBloodstream infection with C. acidovorans in hospital is concentrative and cryptogenic, pathogens were of the same genotype.