ObjectiveTo monitor and analyse the distribution and drug resistance of pathogens isolated from blood culture samples in a hospital , so as to  provide  evidence for the clinical therapy of infections.Methods10 941 blood  samples were detected by BacT/ALERT 3D automated blood culture system , and antimicrobial  susceptibility tests  were performed   with  KirbyBauer disk diffusion method, results were analyzed by WHONET5.4 software.ResultsAmong 10 941 samples, 673 (6.15%) were blood culture positive, 688 pathogens were isolated, 37.94% (261/688) of which were Enterobacteriaceae, 30.67%(211/688) were nonfermentative bacteria, 10.17% (70/688) were Staphylococcus spp.,8.58% (59/688) were Enterococcus spp., 5.23% (36/688) were fungi, 6.54% (45/688) were contaminated bacteria, 0.87%(6/688) were Brucella. The  resistant rate of Escherichia coli to ampicillin/sulbactam, ceftriaxone, cefuroxime, ceftazidime, and ciprofloxacin were 48.27%, 55.17%, 82.76%, 53.10%, and 68.97% respectively; The resistant rate of Acinetobacter baumannii to amikacin, ceftazidime, piperacillin / tazobactam and ciprofloxacin were 35.06%, 38.96%, 32.47%,and 64.94% respectively; The resistant rate of Staphylococcus aureus to erythromycin, clindamycin, levofloxacin were 80.77%, 46.15%, and 75.00% respectively; The resistant rate of Enterococcus feacium to penicillin, erythromycin, rifampicin, and levofloxacin were 94.74%, 97.37%, 97.36%, and 89.47% respectively.ConclusionThe pathogens causing bloodstream infection are widely distributed and highly drug resistant.Detection of clinical specimens of blood culture should conform to the standard in order to avoid contamination and enhance detection quality  .