ObjectiveTo evaluate the endotoxin and bacterial contamination in reverse osmosis (RO) water and dialysate in hospitals in Chongqing.MethodsSpecimens of RO water and dialysate were obtained from 8 hemodialysis units in Chongqing；Bacterial culture was performed and endotoxin was measured with ATi dynamic test tube meter.ResultsBacterial counts of RO water in 8 hospitals were all ≤200 CFU/mL,bacteria counts of dialysate of 1 blood purification centers were>2 000 CFU/mL, the majority of isolated bacteria were gramnegative bacteria；the contents of endotoxin in the RO water of  8 centers were all ＜2 EU/mL.ConclusionRO water and dialysate in most hospitals in Chongqin are conformed to national standard, but bacterial contamination of RO water and dialysate is existing in some Chongqing blood purification centers.The regular detection of endotoxin and bacteria should be undertaken to ensure the safety of medical treatment.