ObjectiveTo realize the condition of nosocomial infection (NI) in Fujian Provincial Hospital, so as to prevent and control NI effectively. MethodsAccording to the survey project of prevalence rate of nationwide NI established by ministry of health, the prevalence rate of NI at a given day between October, 2007 and October, 2009 were conducted.ResultsThe prevalence rate of NI was 5.67%, 6.97% and 4.57% (χ2=8.970, P=0.011), respectively, NI case rate was 6.26%,7.45% and 5.11% (χ2=7.846, P=0.020), respectively, the highest was in 2008，there was significant difference among 3 years. Respiratory tract infection was the most common infection, which accounting for 65.69% , followed by urinary tract (11.11%) and surgical site infetion(5.56%); The departments with high rate of NI were general intensive care unit (30.50%), hematology (24.66%), neurosurgery (17.10%) and neurology （11.31%）; The main pathogen in NI were gramnegative bacilli, which accounting for 66.18% of all pathogens, Acinetobacter baumannii infection showed a rising trend, the constitutional ratio was 18.31%, 20.00% and 21.43% respectively in 2007-2009.ConclusionThe monitor and intervention of key departments and key sites of NI should be strengthened, the enhancement of consciousness of NI among medical staff plays an important role in reducing the occurrence of NI.