ObjectiveTo evaluate the bacteria load and disinfectant efficacy after the opening of rapid hand disinfectant , so as to provide reference for the effective use of rapid hand disinfectant in clinic. MethodsThree bottles of rapid hand disinfectant in the same department and at the same time were opened for using, 15 samples of these disinfectant were randomly taken at the 1st,10th,20th, 30th, and 40th day of opening for bacterial culture, and hand samples disinfected with disinfectant were also taken for bacterial culture. Results At the 1st day of the opening , the highest bacterial count in disinfectant and disinfected hands were 11 CFU/mL and 6 CFU/cm2 respectively, but with the prolonged duration of opening of disinfectant , bacterial count in disinfectant and hands increased obviously, which were up to 93.1 CFU/mL and 9.45 CFU/ cm2 respectively.ConclusionRapid hand disinfectant should be used within 30 days after opening; for the department without enough staffs, smallpackaged rapid hand disinfectant is suggested to avoid waste and contamination.