ObjectiveTo investigate the distribution and drugresistance of Burkholderia cepacia isolated from an intensive care unit (ICU) in a hospital.MethodsBurkholderia cepacia isolated from ICU in a hospital between January 2008 and December 2009 were analysed retrospectively .Results166 strains of Burkholderia cepacia were isolated,which accounting for 23.82% (166/697) of the total isolated strain, 153 of which were isolated from sputum and throat swabs, 9 from urine, 2 from fluid in blood catheter,2 from drainage fluid. Antimicrobial susceptibility test showed that Burkholderia cepacia had high resistance to antimicrobial agents including cefazolin, imipenem, ampicillin/sulbactam,gentamicin,tobramycin, and amikacin, which were up to 98.19%-100.00%; and Burkholderia cepacia had low resistant rate (11.45%) to the thirdgeneration cephalosporins (ceftazidime).The lowest resistant rate (10.84%) was to piperacillin/tazobactam. ConclusionThe isolation of Burkholderia cepacia is high in ICU in this hospital , and resistance to commonly used antimicrobial agents is high , clinicians should perform pathogenic detection, and use antimicrobial agents rationally.