ObjectiveTo analyze the distribution and drug resistance of pathogens in type 2 diabetes mellitus patients combined with urinary system infection, so as to provide reference for rational use of antimicrobial agents. MethodsPathogens were isolated by routine method, and identified with VITEK32 system , drug susceptibility test was carried out by KirbyBauer method, and yeastlike fungi were identified by chromagar.ResultsA total of 256 pathogens were isolated, 150 (58.59%) of which were gramnegative bacilli, 71 (27.74%) were grampositive cocci, 35 (13.67%) were yeastlike fungi. 42.86% of Escherichia coli and 37.78% of Klebsiella pneumoniae were extendedspectrum βlactamaseproducing strains, but sensitive to imipenem, meropenem, piperacillintazobactam, amikacin, cefoxitin and cefepime were still high, the resistant rate was between 0.00%-29.41%. The isolated coagulase negative Staphylococcus, Staphylococcus aureus and Enterococcus were sensitive to vancomycin, linezolid, and chloramphenicol, the resistant rate was between 0.00%-20.00%.ConclusionThe main pathogens in type 2 diabetes mellitus patients combined with urinary system infection is gramnegative bacilli , infection with fungi has a rising tendency. Pathogenic detection and drug susceptibility test should be paid attention.