ObjectiveTo realize the distribution and drugresistance of pathogens isolated from clinical samples in a cardiovascular hospital , so as to provide reference on clinical antiinfection treatment.MethodsThe data of  clinical pathogens  isolated from January to December 2009 were analyzed.ResultsA total of 1 330 pathogenic strains were isolated, including 1 044 (78.49%) gramnegative bacilli ,  215 (16.17%) grampositive cocci, and 71(5.34%) fungi.  Gramnegative bacilli mainly consisted of Klebsiella pneumoniae(308 strains),Pseudomonas aeruginosa (205 strains), Acinetobacter baumannii (138 strains), Enterobacter cloacae(122 strains) ,and Escherichia coli(105 strains). The percentage of ESBLs produced by Escherichia coli and Klebsiella spp. was 79.00% and 41.00% respectively . Imipenem was the most effective antimicrobial agents for treating gramnegative bacilli. 40.00% of Staphylococcus aureus and  98.11% of coagulase negative Staphylococcus were methicillin resistant. No Staphylococcus spp. and Enterococcus spp. strain was found to be resistant to vancomycin.ConclusionRealizing the pathogen and its drug resistance is very important to prevent and control nosocomial infections and rational  antimicrobial use for clinical treatment.