Objective To realize the molecular epidemiology and antimicrobial susceptibility patterns of Pseudomonas aeruginosa (PA)in a children's hospital.MethodsFiftysix  strains of PA isolated  from hospitalized children  were performed antimicrobial susceptibility test and pulsedfield gel electrophoresis( PFGE) genotyping.ResultsAll strains were resistant to ampicillin, resistant rate was 100.00%， and the resistant rate to amikacin and ciprofloxacin were both 0.00%, the resistant rates to the other antimicrobial agents  were between 1.79%-96.43%; isolates were  classified into 18 types by PFGE typing，26 of which were type A (all  were isolated from children in respiratory department )  , 12 were type B(11 strains from endocrinology department), 3 were type C , and 1 was type DR each , the isolated bacteria varied with different department, respiratory tract department had the most isolated strains (27 strains, 48.21%), the next was endocrinology department(13 strains, 23.21%).ConclusionTwo different major epidemic isolates of PA were isolated from  respiratory and endocrinology department in this hospital.  PFGE typing is a reliable method to characterize the prevalence isolates.