ObjectiveTo analyze the pathogen colonization and risk factors of burn wounds in different periods.MethodsExcretion of burn wounds in 131 patients were performed bacterial culture in different periods , relevant data were surveyed retrospectively, pathogen colonization and influencing factors were analyzed.ResultsThe pathogen colonization rate increased gradually at the 3rd，7th，14th day after burn ,which was 26.72%,61.32% and 89.93% respectively, there were significant difference (χ2=71.02, P<0.00), the influence of six risk factors on the pathogen colonization had statistical significance , including unit environment，patients’ age， drug application at wound，combination use of antimicrobial agents , depth and types of burn(all P<0.05).ConclusionThe pathogen colonization rate , multiple drugresistant pathogen constantly increase after burn, it’s very important to prevent burn wound infection by division management for burn patients, rational use of drug , active treatment of wounds, and strict executing of disinfection and isolation measures.