ObjectiveTo investigate the relationship between human parvovirus B19 and spontaneous abortion by detecting human parvovirus B19 DNA and IgM of women in normal pregnancy and spontaneous abortion .MethodsThe blood of women in abortion (observation group, 28 cases) and normal pregnancy (control group, 33 cases) were collected, human parvovirus B19 DNA and IgM were detected with PCR and ELISA.ResultsThe positive rate of human parvovirus B19 DNA was 28.57% (8/28) in observation group and 9.09% (3/33) in control group, there was significant difference between two groups(χ2=3.98, P<0.05); human parvovirus B19 IgM was detected in one sample of observation group(3.57%,1/28), positive samples were not detected in the control group(0.00%).ConclusionHuman parvovirus B19 infection rate is higher in women of spontaneous abortion than in normal pregnant women,suggesting that human parvovirus B19 infection may be one of the causes leading to abortion.