ObjectiveTo survey bacterial antimicrobial resistance status and evaluate the general indexes which can  evaluate the level, trend, degree and rate of antimicrobial resistance. MethodsAntimicrobial sensitivity test results  of 1 523 strains of pathogens isolated from 2 884 patients with nosocomial infection  in a hospital from 2002 through 2009 were analyzed, and change in common bacterial resistance  to antimicrobial agents was described with  antimicrobial resistant rate of stages, rate difference, ratio and sum of the rate difference . ResultsThe increase of antimicrobial resistant rate was the fastest in  Escherichia coli to piperacillin/tazobactam , Pseudomonas aeruginosa to ceftazidime and piperacillin, Klebsiella pneumoniae to amoxicillin/clavulanic acid, and Enterobacter cloacae to ciprofloxacin, the difference  value was 8.43,6.87,  6.80 ,31.36 and 5.65,  respectively; increase of total antimicrobial resistant rate of  Klebsiella pneumoniae exceeded Escherichia coli, sum of the rate difference was 136.53 and 115.65 respectively; and decrease of  total antimicrobial resistant rate of Enterobacter cloacae exceeded Pseudomonas aeruginosa,  sum of the rate difference was -125.16 and -6.15 respectively; total antimicrobial resistant rate of Staphylococcus aureus showed  a trend of  increase, and Staphylococcus epidermidis showed  a trend of decrease, sum of the rate difference was 50.30 and -157.01, respectively.ConclusionFour indexes including antimicrobial resistant rate of stages, rate difference, ratio and sum of the rate difference can evaluate the antimicrobial resistance from many aspects , which  has important guidance for the  study of antimicrobial resistance and antimicrobial application in clinic.