ObjectiveTo evaluate the point prevalence rate and trends of nosocomial infection (NI) in a hospital , so as to provide the basis for targeted prevention of NI.MethodsAccording to the criteria set up by the Ministry of Health, point prevalence rates of NI in patients who were hospitalized on the appointed day were investigated by the combination of bedside examination and medical records checking.Results942 and 929 patients were surveyed on June 18 ,2009 and June 30,2010 respectively, the actual investigated rate was 100.00%; The point prevalence rate of NI was 4.14% (39 cases) and 4.52%（42 cases）respectively, and the point prevalence rate of casetimes was 5.10%（48 cases） and 5.17%（48 cases）respectively；  The high infected sites were lower respiratory tract （56.25% vs 58.34%） and urinary tract（16.66% vs 20.83%）; The main risk factors of NI were male patients, patients aged ＜2  or ＞ 60 years of age , urinary catheter , blood vessel catheter, use of ventilator; The usage rate of antimicrobial agents was  54.67% and 52.31% respectively, sample detection rate was 42.30% and 43.30% respectively.ConclusionTargeted surveillance and management of key departments and key sites should be strengthened; the monitoring and management of antimicrobial agents should be stressed,  antimicrobial usage rates should be reduced,  detection rate of samples should be improved, and antimicrobial agents should be used correctly and  rationally.