ObjectiveTo analyze 8 strains of Klebsiella pneumoniae isolated from the surgical ward in a hospital by pulsedfield gel electrophoresis(PFGE), so as to provide scientific evidence for the control of nosocomial infection. MethodsAntimicrobial susceptibility test of Klebsiella pneumoniae was performed by microbroth dilution and KirbyBauer method, genotyping was performed with PFGE.ResultsAntimicrobial susceptibility test showed that except the strain Kp1, 7 clinical strains were the same sensitivity to 11 antimicrobial agents, and they were all multidrug resistant； PFGE showed that 7 strains were the same pattern. ConclusionSeven of 8 Klebsiella pneumoniae strains came from the same clone, which suggesting it might be an outbreak of nosocomial infection.