ObjectiveTo evaluate the distribution and antimicrobial resistant characteristics of pathogens isolated from female patients with urinary tract infection (UTI).MethodsPathogens isolated from urine of female patients with UTI were identified and performed antimicrobial susceptibility test from January 2008 to October 2010.ResultsA total of 182 strains of pathogens were isolated and the top six pathogens were Escherichia coli(46.70%), Staphylococcus spp.(13.74%), Enterococcus spp.(9.34%), Klebsiella pneumoniae(8.24%), Pseudomonas aeruginosa（4.95%）, and fungus（4.95%）. 22.35% of Escherichia coli and 26.67% of Klebsiella pneumoniae produced extendedspectrum βlactamases; 50% of coagulasenegative Staphylococcus and 44.44% of Staphylococcus aureus were methicillinresistant. Isolated bacteria had different resistance to antimicrobial agents, and were multidrugresistant.ConclusionPathogens and antimicrobial resistance detection in urinary infection is important for controlling drugresistant strains transmission and guide rational use of antimicrobial agents.