ObjectiveTo evaluate the effect of continuous hand hygiene quality improvement on healthcareassociated infection. MethodsHand washing facilities were installed and equipped with liquid soap and hand disinfection in 2007. Hand hygiene quality was enforced through target management and supervision and feedback. ResultsFrom 2007 to 2010, hand washing facilities improved continuously, handdry method changed from hand dryer to paper towel; the number of departments with hand washing liquid and hand disinfection consumption of 20 mL/bed day increased from 4 in 2009 to 17 in 2010, the number of departments with hand washing liquid and hand disinfection consumption of 10-20 mL/bed day increased from 13 in 2009 to 47 in 2010; the qualified rate of hand hygiene increased from 93.93% in 2008 to 98.67% in 2010; healthcareassociated infection rate decreased from 2.74% in 2008 to 1.73% in 2010.ConclusionContinuous hand hygiene quality can improve the healthcare workers’ compliance to hand washing, and decrease healthcareassociated infection.