ObjectiveTo compare the effect of three disinfection and cleaning methods on neonatal incubators.MethodsAfter terminal disinfection, neonatal incubators were randomly divided into three groups, and were wiped respectively with 500 mg/L available chlorine(group Ⅰ), 250 mg/L available chlorine (group Ⅱ), and water (group Ⅲ) once a day, samples of incubators were taken for bacterial culture immediately after drying and 24 hours after treating, monitor were performed for consecutive 6 days, disinfection or cleaning effect of 3 groups were compared. ResultsThe qualified rate in group Ⅰ, Ⅱ and Ⅲ was 100.00% (36/36), 97.40% (75/77), and 96.67% (58/60) respectively when samples were taken immediately; the qualified rate in group Ⅰ, Ⅱ and Ⅲ was 91.67% (33/36), 94.81% (73/77), and 96.67% (58/60) respectively when incubator samples were taken 24 hours after treating (all P>0.05). ConclusionThe above three methods can all meet the environmental hygiene requirements of neonatal units, but water cleaning method is more simple, economical, and environmental protective, which is worth popularizing.