ObjectiveTo evaluate the effect of three different nursing intervention methods on preventing ventilatorassociated pneumonia(VAP).MethodsNinety intensive care unit(ICU) patients with mechanical ventilation were divided into three groups according to the time of admission, 30 cases were performed routine ventilation care (routine group) from July 2007 to December 2007; 30 cases used heat and moisture exchanger (HME group) when receiving ventilation from January 2008 to June 2008; 30 cases used heat and moisture exchanger and received bundle care of nursing (comprehensive intervention group) from July 2008 to December 2008. The days of ventilation and ICU stay, incidence of VAP, and pathogens causing VAP were compared among three groups.ResultsCompared with routine and HME group, mechanical ventilation time shortened obviously in comprehensive intervention group (［8.83±6.65］d vs ［16.17±4.87］ d, t=5.43,P=0.00；［8.83±6.65］ d vs ［12.43±5.27］ d，t=2.31,P＜0.05); Compared with routine group, time stay in ICU shortened in comprehensive intervention group (［9.30±6.20］d  vs ［14.97±11.35］ d, t=2.42,p<0.05), and the incidence of VAP declined (16.67% vs 43.33%, χ2=5.08, P＜0.05). Twentyseven strains of pathogens were isolated from VAP patients, 48.15% (13/27) were methicillinresistant Staphylococcus aureus, 18.52% (5/27) were Pseudomonas aeruginosa, 14.81%(4/27) were Klebsiella pneumoniae, 7.41%(2/27) were Escherichia coli, and 3.70% (1/27) were Candida tropicalis,Candida albicans and Acinetobacter baumannii (1/27) each.ConclusionHeat and moisture exchanger with bundle care intervention can shorten the duration of mechanical ventilation and time in ICU stay, promote standardized nursing practice, and reduce the incidence of VAP.