ObjectiveTo analyze the distribution and antimicrobial resistance of Staphylococcus aureus (SA) and methicillinresistant Staphylococcus aures（MRSA） isolated from clinical samples in a hospital.MethodsSA isolated from 2007 to 2010 were identified with automatic microorganism analyzer (VITEK2Compact); antimicrobial susceptibility testing was performed by disk diffusion method, and the data were analyzed with WHONET5.5.ResultsA total of 689 SA isolates were isolated within 4 years, 347 (50.36%) of which were MRSA. Of all SA strains, 119 (17.27%) were isolated from intensive care unit (ICU), 109 (15.82%) were from neurosurgery department. Of all MRSA strains, 63 (18.16%) were isolated from neurosurgery department and 61(17.58%）were from ICU; 79.49%（31/39）of SA from respiratory department were MRSA; 73.49% (225 isolates) of MRSA were from sputum, and 12.97% (45 isolates）from wound secretion. MRSA were resistant to most antimicrobial agents, the resistant rate to trimethoprimsulfamethoxazole was 21.1%-42.7%, and there was a decreasing tendency in resistance ; methicillinsensitive Staphylococcus aureus (MSSA) were sensitive to the majority of antimicrobial agents except penicillin; No strains were found resistant to vancomycin in this study.ConclusionIsolation of MRSA is high in this hospital, monitoring of drugresistance of SA should be strengthened, patients with MRSA should be strictly isolated, hand hygiene of health care workers should be stressed, antimicrobial agents should be used rationally, so as to prevent and control the emergence of MRSA.