ObjectiveTo detect the expression of CD81 and low density lipoprotein receptor (LDLR) mRNA in cultured human trophoblast cells from different placental villi, and to explore HCV entry mechanisms.MethodsCD81 and LDLR mRNA expression in trophoblast cells from human placental villi was detected by RTPCR, and expression of CD81 and LDLR was also detected during different pregnancy stages by immunofluorescene method.ResultsCD 81 and LDLR were found in different placental villi and their expression has shown an increasing trend during different stages of gestation by immunofluorescene. ConclusionThe localization and expressions of HCV entryrelated receptor CD 81 and LDLR are found in cultured human trophoblast cells, which lay the foundation for further exploring the molecular mechanism of HCV infection transmission by CLEC4M.