ObjectiveTo observe in vitro inhibitory activity of the metabolites of Pseudomonas aeruginosa (P. aeruginosa) against Candida spp. . MethodsIn vitro inhibitory activity of P. aeruginosa against 54 Candida spp. isolates was detected by crossstreak assay. Results The metabolites of P. aeruginosa had the strongest inhibitory activity on Candida albicans (C. albicans) and Candida glabratas (C. glabratas), and produced the widest zone of inhibition. The inhibition rate of aquamarine pigmentproducing No.6 P. aeruginosas against C. albicans and Candida tropicalis (C. tropicalis) were both 100%, and the inhibition rate of yellow green pigmentproducing No. 8 P. aeruginosas against C. albican,C.tropicalis and Candida krusei (C. krusei) were all 100%,too. The pigmented strains were found to have stronger antifungal activity than the unpigmented strains. ConclusionThe metabolites of P. aeruginosa has strong antifungal activity against C. albicans and C. glabrata.