ObjectiveTo investigate the Klebsiella pneumoniae carbapenemase (KPC) production in clinical isolates of Enterobacteriaceae in a hospital.Methods1 801 clinical Enterobacteriaceae isolates were collected from this hospital from 2009 to 2010. Highly drugresistant strains were screened by antimicrobial susceptibility testing, KPCtype carbapenemase was detected with the modified Hodge testing and polymerase chain reaction (PCR), and DNA sequencing was performed to confirm carbapenemase genotypes.ResultsOf 1 801 Enterobacteriaceae isolates, 783(43.48%) were resistant to the third cephalosporins, 4 of which were also resistant to carbapenem; 2 isolates were screened as drugresistant strains by Hodge testing, and blaKPC2 gene was identified in this 2 isolates by PCR.ConclusionKPC2 Enterobacteriaceae strains with carbapenemaseproducing drugresistant gene have emerged in this hospital, monitor and control should be strengthened.