ObjectiveTo investigate the current status of multidrugresistant organism (MDRO) infection in a hospital, and evaluate intervention strategies in prevention and control of MDRO infection.MethodsMonitor on MDRD infection was conducted, infectionrelated factors were analyzed, administration strategies were put forward, and intervention was implemented.ResultsFrom July 2010 to November 2011, 17 240 patients were admitted in this hospital, 213(1.23%) cases of MDRO infection developed, the major infection sites were respiratory tract (108 cases, 50.70%) and urinary tract (53 cases, 24.88%). MDRO infection rate dropped from 1.49% (93/6 256) before intervention (JulyDecember, 2010) to 1.09%(120/10 984) after intervention (JanuaryNovember, 2011) (χ2=5.07, P=0.02), and constituent ratio of multidrugresistant Pseudomonas aeruginosa dropped from 22.58% to 10.83%; constituent ratio of respiratory tract infection dropped from 59.14% to 44.17%; MDRO infection rate in intensive care unit and tumor internal medicine department dropped significantly (49.02% vs 28.44%, χ2=6.47, P=0.01; 3.72% vs 1.21%, χ2=5.04, P=0.03).ConclusionEffective prevention and control of MDRO infection can be achieved through accurate and timely obtaining information, improving hand hygiene compliance, strengthening training on MDRO infection knowledge, implementing strict disinfection and isolation measures, strengthening nursing care, and using antimicrobial agents rationally.