ObjectiveTo investigate species and drugresistance of pathogens in neonatal septicemia, and provide the basis for clinical treatment.MethodsAnalysis was made on positive blood culture and antimicrobial susceptibility testing results of pathogens from 158 neonates with septicemia who were admitted in a hospital from January 2007 to December 2010.ResultsThe top 3 bacteria in blood culture were coagulasenegative Staphylococcus(CoNS, 77 isolates,48.73%), Staphylococcus aureus (S. aureus,44 isolates,27.84%) and Escherichia coli (E. coli,19 isolates,12.03%); S.aureus and CoNS had high resistant rates to penicillin and erythromycin, but had no resistance to vancomycin and teicoplanin; E.coli had high resistance to ciprofloxacin, cefotaxime,trimethoprim/sulfamethoxazole, but had low resistance to tetracycline, cefoperazone/sulbactam, all were sensitive to imipenem and meropenem.ConclusionStaphylococcus spp. and E. coli are the major pathogens in neonatal septicemia, detection of antimicrobial resistance and choice of rational antimicrobial agents for treatment should be stressed.