ObjectiveTo explore the effect of central line bundle (CLB) on the prevention of central venous catheterrelated bloodstream infection （CRBSI） in an intensive care unit(ICU).MethodsA combination method of retrospective and prospective survey was adopted to compare and analyze the occurrence of CRBSI in two groups of patients, 179 patients in treatment group with central venous catheters (CVCs) from March 2011 to February 2012 implemented CLB, 198 patients in control group with CVCs from January 2010 to December 2010 didn't implement CLB.ResultsThe incidence of CRBSI in treatment group was significantly lower than control group (3.34‰ vs 9.42‰;χ2=5.340,P=0.021); the length of hospital stay was significantly shorter than that in control group(［26.43±7.16］d vs［33.25±8.51］d;t=8.35,P＜0.001）,the onset of CRBSI was significantly later than that of control group (［10.11±2.34）］d vs（6.23±1.92）d; t=17.703,P＜0.001）, the consumption of hand disinfectant increased significantly in treatment group (41.2 mL/ patients day vs 3.5 mL/ patients day；t=34.469,P<0.001）.ConclusionThe implementation of central line bundle in ICU can effectively reduce the incidence of CRBSI, and is helpful for the prevention and control of CRBSI.