ObjectiveTo evaluate the prevalence of healthcareassociated infections(HAIs) in 15 hospitals in Changzhou, so as to provide basis for making policy for preventing and controlling HAIs.MethodsOn August 30, 2011, HAIs in 15 hospitals in Changzhou were surveyed by combination of bedside checking and medical record reviewing.Results9 695 patients should be surveyed, 9 691 (99.96%) were actually surveyed,362 patients developed 382 episodes of HAIs, the prevalence rate and case infection rate was 3.74% and 3.94% respectively. A total of 213 pathogenic isolates were detected, 70.42% were gramnegative bacteria, 17.37% were grampositive bacteria, 11.27% were fungi， and 0.94% were Mycoplasma. The highest prevalence of department was intensive care unit (38.98%). The top five infection sites were lower respiratory tract (43.72%),upper respiratory tract (14.14%),urinary system (11.26%),surgical sites (9.69%), and skin and soft tissue (5.50%).High risk factors for HAIs included the use of ventilator(RR 95%CI,11.37-23.34), vascular access (RR 95%CI,3.91-6.85), and urinary catheterization (RR 95%CI,3.57-5.79). A total of 3 562 patients used antimicrobial agents,the daily antimicrobial usage rate was 36.76%, 1 831 (51.40%) of whom received curative use of antimicrobials, and 1 651 (46.35%) received prophylactic use, 80(2.25%) used antimicrobial agents without indication.ConclusionPrevalence survey of HAIs is helpful for realizing the occurrence of HAI, improving the accuracy of surveillance,recognizing the risk factors for HAI and multidrugresistance bacterial infection, and strengthening the targeted surveillance on the key departments.