ObjectiveTo study the multilocus sequence characteristics of polymerase chain reaction (PCR) map of 3 isolates of methicillinresistant Staphylococcus aureus (MRSA) which carried a new staphylococcal cassette chromosome mec(SCCmec) .MethodsIn the previous PCR typing study on 58 MRSA isolates, 3 (5.17%) were found to carry a new SCCmec multilocus sequence containing 5 bands, which were band A,F,H, B and M. The above amplified products were sequenced, and then compared with the sequences of SCCmec type saved in the international GenBank and the Basic Local Alignment and Search Tool (BLAST）.ResultsThe homology between the detected sequence and the known sequence was over 97%.ConclusionIt’s a new PCR pattern of multilocus sequence of SCCmec type, and maybe the recombinant of conventional SCCmec type I,II and III, which is not only a different PCR pattern, but also has multilocus mutation.