ObjectiveTo evaluate multidrugresistant bacterial (MDRB) healthcareassociated infection (HAI) in malignant tumor patients, so as to provide basis for the effective prevention and control measures.MethodsData on MDRB isolated from various specimens in Henan Tumor Hospital in JanuaryDecember 2011 were analyzed retrospectively.ResultsA total of 2 562 pathogenic strains were isolated, 449 (17.53%) of which were MDRB. Of all MDRB，305 （67.93%）were gramnegative bacteria,the isolation rate of Escherichia coli was the highest （54.57%，245 isolates）, followed by Klebsiella pneumoniae (9.80%，44 isolates);grampositive bacteria were 144 isolates（32.07%），the isolation rate of Staphylococcus aureus (11.80%，53 isolates) and Staphylococcus epidermidis (11.58%，52 isolates) were higher. MDRB infections were mainly concentrated on general surgery (26.73%，120 isolates)and hepatobiliary surgery（21.83%, 98 isolates); the major specimen was drainage fluid of pleuroperitoneal cavity(39.42%), followed by sputum （19.82%）and blood（19.16%）.ConclusionHAI monitoring helps to realize MDRB infection and prevent outbreak of HAI.