ObjectiveTo investigate distribution and genotypes of human papillomavirus (HPV) infection，as well as relationship between HPV infection and female genital diseases in Fengtai District of Beijing.MethodsHPV detection was performed among 673 female patients with genital diseases in an outpatient department from September 1 to 30, 2012, the relationship between HPV genotypes and genital diseases was analyzed.Results Of 673 patients, 140(20.80%) were with positive HPV test; HPV detection rate in patients with cervicitis, cervical lesions, cervical cancer,and other inflammatory diseases （including vulvitis, vaginitis, pelvic inflammatory disease, and urinary tract infection）was 23.32%（87 cases）,38.89%（14 cases）,61.11%（11 cases）, and 11.38%（28 cases） respectively. 140 patients were detected 197 times of HPV, 13 subtypes were included（type 52,16,58、56,39,51,68,33,59,18,31,35,and 45）, the top three HPV types were ranked by HPV52（18.78%）, HPV16（16.75%） and HPV58 （11.67%）; Incidence of singleinfection was 73.57% (103 cases),double and multiple infection was 26.43%(37 cases), all HPVpositive patients with cervical cancer had double infection.ConclusionThe incidence and types of HPV infection in local female patients are regionally distributed. Survey on the incidence and distribution can provide epidemiological data for exploring effective prevention and therapy for HPVrelated diseases.