(S.aureus) isolated from a hospital.MethodsSpecimen sources and antimicrobial susceptibility test results of 520 S.aureus isolates from a hospital between January 2011 and August 2012 were analyzed.ResultsOf 520 S.aureus isolates, isolation rate of methicillinresistant S.aureus (MRSA) was 23.27%(121/520), mainly from sputum specimen (80.82%), followed by urine(33.33%), blood(25.00%) and drainage(18.31%), the detection rate was the lowest in skin and soft tissue (11.38%). Vancomycin and linezolidresistant S. aureus was not found; S.aureus was sensitive to vancomycin, linezolid,compound sulfamethoxazole and furantoin. The sensitive rates of MRSA from skin and soft tissue to gentamycin, levofloxacin, ciprofloxacin,moxifloxacin,rifampicin and tetracycline were higher than those from the other specimen sources(P<0.05), but sensitive rates to erythromycin and clindamycin were lower than those from the other specimen sources(P<0.05). MRSA was totally resistant to penicillin, MRSA from communityassociated infection was more sensitive to gentamycin, levofloxacin, ciprofloxacin,moxifloxacin， rifampicin and tetracycline than that from healthcareassociated infection (HAI) (P<0.05)， but susceptible rates to erythromycin, clindamycin and compound sulfamethoxazole were lower than that from HAI (P<0.05).ConclusionS. aureus is an important pathogen isolated from this hospital, and with high antimicrobial resistance, it is important to use antimicrobial agents rationally according to antimicrobial susceptibility test result.