ObjectiveTo analyze the distribution and antimicrobial resistance of common pathogens isolated from a hospital，and provide reference for rational antimicrobial use.MethodsData of 2 208 pathogens from clinical specimens from January 2011 to December 2012 were analyzed retrospectively by WHONET5.5 software.ResultsA total of 2 208 pathogens were isolated，pathogens were mainly isolated from sputum (50.63%, 1 118/2 208）and urine (22.69%, 501/2 208). 1 418(64.22%) isolates were gramnegative bacteria, Escherichia coli was the most common gramnegative bacillus (15.72%), followed by Klebsiella pneumoniae(11.96%), Acinetobacter baumannii(10.64%), and Pseudomonas aeruginosa(9.83%); 577(26.13%) isolates were grampositive bacteria, 11.23% of which were Staphylococcus aureus; 213(9.65%) isolates were fungi.Gramnegative bacilli were sensitive to imipenem,but resistance of Acinetobacter baumannii was serious（resistant rate was 55.74%-74.04%）.The percentage of methicillinresistant Staphylococcus aureus and methicillinresistant coagulase negative Staphylococcus was 69.76% and 87.56% respectively, no Staphylococcus spp. isolates was found to be resistant to vancomycin.ConclusionThe main infection site of patients in this hospital is respiratory tract; antimicrobial resistance of pathogens is serious, intensified surveillance on antimicrobial resistance is important to guide rational use of antimicrobial agents and reduce multidrug resistant strain.